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human pd1 fc protein  (R&D Systems)


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    R&D Systems human pd1 fc protein
    DA treatment sensitises MDA‐MB‐231 cells to activated T cells. Representative images (A) and quantification (B) of green fluorescence‐labelled <t>PD1/Fc</t> on DA‐treated MDA‐MB‐231 cells for 24 h. Representative images (C) and quantification (D) of crystal violet‐stained live cancer cells in T cell‐mediated cancer cell killing assay. MDA‐MB‐231 cells were then co‐cultured with activated T cells for 48 h with or without DA (12.5 μM) and subjected to crystal violet staining. Statistical significance was determined using * p < 0.05 and ** p < 0.01. All error bars are expressed as mean ± SD of three independent experiments. Abbreviations: DA, dihydroartemisinin; CTRL, negative control.
    Human Pd1 Fc Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pd1+fc+protein/Recombinant+Human+PD-1+Fc+Chimera+Protein%2C+CF/pmc12790935-54-15-19
    Average 96 stars, based on 95 article reviews
    human pd1 fc protein - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "FoxO3a‐Mediated Modulation of PD‐L1 Expression and Inhibition by Dihydroartemisinin in Triple‐Negative Breast Cancer"

    Article Title: FoxO3a‐Mediated Modulation of PD‐L1 Expression and Inhibition by Dihydroartemisinin in Triple‐Negative Breast Cancer

    Journal: Journal of Cellular and Molecular Medicine

    doi: 10.1111/jcmm.70947

    DA treatment sensitises MDA‐MB‐231 cells to activated T cells. Representative images (A) and quantification (B) of green fluorescence‐labelled PD1/Fc on DA‐treated MDA‐MB‐231 cells for 24 h. Representative images (C) and quantification (D) of crystal violet‐stained live cancer cells in T cell‐mediated cancer cell killing assay. MDA‐MB‐231 cells were then co‐cultured with activated T cells for 48 h with or without DA (12.5 μM) and subjected to crystal violet staining. Statistical significance was determined using * p < 0.05 and ** p < 0.01. All error bars are expressed as mean ± SD of three independent experiments. Abbreviations: DA, dihydroartemisinin; CTRL, negative control.
    Figure Legend Snippet: DA treatment sensitises MDA‐MB‐231 cells to activated T cells. Representative images (A) and quantification (B) of green fluorescence‐labelled PD1/Fc on DA‐treated MDA‐MB‐231 cells for 24 h. Representative images (C) and quantification (D) of crystal violet‐stained live cancer cells in T cell‐mediated cancer cell killing assay. MDA‐MB‐231 cells were then co‐cultured with activated T cells for 48 h with or without DA (12.5 μM) and subjected to crystal violet staining. Statistical significance was determined using * p < 0.05 and ** p < 0.01. All error bars are expressed as mean ± SD of three independent experiments. Abbreviations: DA, dihydroartemisinin; CTRL, negative control.

    Techniques Used: Fluorescence, Staining, Cell Culture, Negative Control

    Related Articles

    Incubation:

    Article Title: Disruption of SIRT7 Increases the Efficacy of Checkpoint Inhibitor via MEF2D Regulation of Programmed Cell Death 1 Ligand 1 in Hepatocellular Carcinoma Cells.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: FoxO3a‐Mediated Modulation of PD‐L1 Expression and Inhibition by Dihydroartemisinin in Triple‐Negative Breast Cancer
    Article Snippet: .. Cells in each group were fixed in 4% PFA for 15 min, incubated with recombinant human PD1 Fc protein (R&D Systems, Minneapolis, MN, USA) for 1 h, and then incubated with anti‐human Alexa Fluor 488 secondary antibodies (Thermal Fisher Scientific) for 1 h at room temperature. .. Nuclei were stained with DAPI, and cells were imaged using a Nikon A1R confocal microscope.

    Recombinant:

    Article Title: Disruption of SIRT7 Increases the Efficacy of Checkpoint Inhibitor via MEF2D Regulation of Programmed Cell Death 1 Ligand 1 in Hepatocellular Carcinoma Cells.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: FoxO3a‐Mediated Modulation of PD‐L1 Expression and Inhibition by Dihydroartemisinin in Triple‐Negative Breast Cancer
    Article Snippet: .. Cells in each group were fixed in 4% PFA for 15 min, incubated with recombinant human PD1 Fc protein (R&D Systems, Minneapolis, MN, USA) for 1 h, and then incubated with anti‐human Alexa Fluor 488 secondary antibodies (Thermal Fisher Scientific) for 1 h at room temperature. .. Nuclei were stained with DAPI, and cells were imaged using a Nikon A1R confocal microscope.



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    DA treatment sensitises MDA‐MB‐231 cells to activated T cells. Representative images (A) and quantification (B) of green fluorescence‐labelled <t>PD1/Fc</t> on DA‐treated MDA‐MB‐231 cells for 24 h. Representative images (C) and quantification (D) of crystal violet‐stained live cancer cells in T cell‐mediated cancer cell killing assay. MDA‐MB‐231 cells were then co‐cultured with activated T cells for 48 h with or without DA (12.5 μM) and subjected to crystal violet staining. Statistical significance was determined using * p < 0.05 and ** p < 0.01. All error bars are expressed as mean ± SD of three independent experiments. Abbreviations: DA, dihydroartemisinin; CTRL, negative control.
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    DA treatment sensitises MDA‐MB‐231 cells to activated T cells. Representative images (A) and quantification (B) of green fluorescence‐labelled <t>PD1/Fc</t> on DA‐treated MDA‐MB‐231 cells for 24 h. Representative images (C) and quantification (D) of crystal violet‐stained live cancer cells in T cell‐mediated cancer cell killing assay. MDA‐MB‐231 cells were then co‐cultured with activated T cells for 48 h with or without DA (12.5 μM) and subjected to crystal violet staining. Statistical significance was determined using * p < 0.05 and ** p < 0.01. All error bars are expressed as mean ± SD of three independent experiments. Abbreviations: DA, dihydroartemisinin; CTRL, negative control.
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    Image Search Results


    DA treatment sensitises MDA‐MB‐231 cells to activated T cells. Representative images (A) and quantification (B) of green fluorescence‐labelled PD1/Fc on DA‐treated MDA‐MB‐231 cells for 24 h. Representative images (C) and quantification (D) of crystal violet‐stained live cancer cells in T cell‐mediated cancer cell killing assay. MDA‐MB‐231 cells were then co‐cultured with activated T cells for 48 h with or without DA (12.5 μM) and subjected to crystal violet staining. Statistical significance was determined using * p < 0.05 and ** p < 0.01. All error bars are expressed as mean ± SD of three independent experiments. Abbreviations: DA, dihydroartemisinin; CTRL, negative control.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: FoxO3a‐Mediated Modulation of PD‐L1 Expression and Inhibition by Dihydroartemisinin in Triple‐Negative Breast Cancer

    doi: 10.1111/jcmm.70947

    Figure Lengend Snippet: DA treatment sensitises MDA‐MB‐231 cells to activated T cells. Representative images (A) and quantification (B) of green fluorescence‐labelled PD1/Fc on DA‐treated MDA‐MB‐231 cells for 24 h. Representative images (C) and quantification (D) of crystal violet‐stained live cancer cells in T cell‐mediated cancer cell killing assay. MDA‐MB‐231 cells were then co‐cultured with activated T cells for 48 h with or without DA (12.5 μM) and subjected to crystal violet staining. Statistical significance was determined using * p < 0.05 and ** p < 0.01. All error bars are expressed as mean ± SD of three independent experiments. Abbreviations: DA, dihydroartemisinin; CTRL, negative control.

    Article Snippet: Cells in each group were fixed in 4% PFA for 15 min, incubated with recombinant human PD1 Fc protein (R&D Systems, Minneapolis, MN, USA) for 1 h, and then incubated with anti‐human Alexa Fluor 488 secondary antibodies (Thermal Fisher Scientific) for 1 h at room temperature.

    Techniques: Fluorescence, Staining, Cell Culture, Negative Control